Real Time PCR
UAGC offers 3 Cyclers for Quantitative or Real Time PCR. After a brief training, local users can schedule time on the instrument and run their own samples. Real Time PCR is also utilized as one of the platforms in our genotyping services.
Users provide their own consumables when using the instruments.
Real Time PCR provides a quantitative assessment of your sample for gene expression, gene transcription studies or targeted nucleic acids.
Sample Preparation Details
For a database of optimized Real-time PCR primers please click on the following link: http://www.realtimeprimers.org
Local users may browse our instruments available to schedule. To schedule an instrument, please use our iLab resource schduler
- ABI7300 (LSS or Keating Building)
- ABI7900: Accomodates 384 well plates (Keating Building)
Schedule time on an instrument-
Academic (UA): $25.00 | Academic (non-UA): $30.00| Industry: $37.50 Unit: Per Hour- Unassisted use
SNP Genotyping- Service-
Academic (UA): $3.75 | Academic (non-UA): $4.50 | Industry: $5.63 Unit: Per reaction*
Gene Expression- Service-
Academic (UA): $4.25 | Academic (non-UA): $5.10 | Industry: $6.38 Unit: Per reaction*
*Note: Assays will be billed to the researcher. Contact UAGC prior to your submission to discuss the ordering of assay reagents.
Varies by project
Users will assume the cost (in-whole) of assay-specific consumables. UAGC is happy to order the materials from our preferred manufacturer at the most appropriate scale for your project.