Nanostring nCounter

The UAGC offers gene expression services using the NanoString nCounter® Analysis System. This system uses molecular "barcodes" and single-molecule imaging to profile up to 800 mRNAs, microRNAs, or DNA targets simultaneously with high sensitivity and precision, without any reverse transcription or amplification.

General Information


Sample Input

Gene Expression, miRNA and miRGE Assasys:
        •  100ng purified total RNA or 300ng degraded
Copy Number Variation:
        •  300ng purified genomic DNA

The UAGC offers a variety of extraction services for most sample types, please inquire for details and pricing.

Expected Results

Gene Expression
Limit of Detection:
        •  0.5fM spike-in control (~1 copy per cell); 90% of the time
Fold-change Sensitivity:
        •  >1.5 fold (>5 copies per cell)
        •  >2 fold change (>1 copy per cell)
miRNA Assay
Limit of Detection:
        •  ≤ 0.5fM (~10 copies per cell)
Fold-change Sensitivity:
        • > 2 fold change
Copy Number Variation
Reproducibility:
        •  99%
Number of Copies Detected:
        •  0-4, multiallelic
miRGE Assays
Limits of Detection:
        •  For miRNA: >2.5 copies per cell copy per cell
        •  For mRNA: 1 copy per cell
Fold-change Sensitivity:
        •  >2 fold change

Details

Sample Preparation Details

Varies, please inquire.

Workflow

A variety of pre-designed panels are available for off-the-shelf use. Each cartridge can run up to 12 samples, and most panel kits are sold with chemistry for 12, 24, 48 or 96 samples (Custom Panels start at 48 samples). For a complete list of the number of samples and specific genes covered by each panel, please visit the following links:
 

Oncology Panels

  • Breast Cancer 360™ Panel
  • PanCancer IO 360™ Panel
  • CAR-T Characterization Panel
  • Metabolic Pathways Panel
  • Hallmarks of Cancer Collection:
    • PanCancer Pathways Panel
    • PanCancer Immune Profiling Panel
    • PanCancer Progression Panel
  • PlexSet™ Pre-selected Panels
  • Vantage 3D™ RNA Panels
  • Vantage 3D™ Gene Fusion Panels
  • miRNA Panels
  • nCounter Gene Fusion Panels (Ex-US only)

 

Immunology Panels

  • Autoimmune Profiling Panel
  • Autoimmune Discovery Panel
  • Metabolic Pathways Panel
  • Fibrosis Panel
  • Human Organ Transplant Panel
  • Immunology Panels
  • Inflammation Panels
  • miRNA Panels
  • Myeloid Panels
  • NHP Immunology Panel

 

Neuroscience Panels

  • Neuropathology Panels
  • Neuroinflammation Panels
  • Mouse and Human AD Panels
  • miRNA Panels
  • PlexSet™ Pre-selected Panels

Gene Fusion Panels and CNV Assays

Custom CodeSets
For ultimate flexibility, you can work with NanoString directly (consultation is free of charge) to design a custom Gene Expression or Copy Number Variation code set tailored exactly to your research needs. This is especially useful for organisms that don't already have pre-built panels, hard-to-target sequences, and splice variation and fusion transcript detection. The custom CodeSet design process begins by selecting a list of targets and submitting them to NanoString. Once the design is approved by you, their Manufacturing Group will assemble your CodeSet, perform extensive quality control testing, and ship all components directly to the UAGC for processing. Custom CodeSet pricing is matrix based, with the price per data point declining with increasing numbers of genes and numbers of samples assayed.
miRGE Code Sets
Two assays, one reaction- Use this method to detect subsets of both mRNAs and miRNAs across all biological levels of expression. The nCounter miRGE Assay delivers accurate and sensitive profiling of 100 to 200 mRNA targets and between 5 and 30 miRNA targets, all in a single tube reaction.

Price

Academic (UA): $575.00 | Academic(non-UA): $690.00 | Industry: $862.50 | Unit: Cartridge

Note- Cost per Cartridge does not include QC, additional labor or NanoString Code Sets. Reagents must be purchased separately. Please inquire for more details.

Turnaround Time

Depends on assay type; please inquire.

Related Services

Sample Prep and QC